Reporter

Part:BBa_K929301:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)


Potsdam Standard Backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 699
    Illegal AgeI site found at 811
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To create the Potsdam Standard Backbone, we amplified the RFP expression cassette (BBa_J04450) to add the restriction site for Apa I and Sph I. Then, the amplified expression cassette was insert into pSB1C3 using Xba I and Pst I.


Source

BBa_J04450

References